phenomaster indirect calorimetry system Search Results


calorimetry system  (TSE systems)
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TSE systems calorimetry system
Acute fasting-mediated alterations in BAT of mildly cold-stressed mice. (A) Number of up- and downregulated (1.5 x, FDR5) genes in BAT of 24 h fasted mice. (B) GSEA Hallmark analysis results with top five pathways with highest or lowest normalized enrichment scores (NES). Numbers of mapped genes are shown in the bars. (C) BAT mRNA expression of genes encoding for browning markers of ctrl, 24 h fasted, and 4 h refed mice. (D) Ucp1 mRNA and (E) UCP1 protein expression (from WES digital western blotting) in BAT harvested after 1, 3, 6, 12, and 24 h of fasting with samples from circadian-matched ad libitum fed controls. GAPDH served as loading control. (F) Representative images (magnification, ×100) of BAT of ctrl and 24 h fasted mice stained with hematoxylin and eosin (E–H) and immunohistochemistry with anti-UCP1 antibody. (G) Cell size of was analyzed by using ImageJ and indicated as square pixels (px 2 ). (H) BAT weight in grams (g) of ctrl and 24 h fasted mice. (I,J) Respiratory exchange ratio (RER), (K,L) energy expenditure (EE), and (M,N) motility of ctrl and 24 h fasted mice as validated by indirect <t>calorimetry</t> in metabolic cages. Data are presented as mean values ± SEM. Significant differences in the mRNA expression of fed, fasted, and refed mice were analyzed by 2-way ANOVA and Tukey’s multiple comparisons test, for BAT weight, RER and energy expenditure unpaired t - test was performed. Differences not indicated with asterisks or indicated with ns are not statistically significant ( p > 0.05). *** p < 0.001, ** p < 0.01, and * p < 0.05.
Calorimetry System, supplied by TSE systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phenomaster+indirect+calorimetry+system/PhenoMaster/pmc09207587-168-9-13
Average 96 stars, based on 1 article reviews
calorimetry system - by Bioz Stars, 2026-09
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Acute fasting-mediated alterations in BAT of mildly cold-stressed mice. (A) Number of up- and downregulated (1.5 x, FDR5) genes in BAT of 24 h fasted mice. (B) GSEA Hallmark analysis results with top five pathways with highest or lowest normalized enrichment scores (NES). Numbers of mapped genes are shown in the bars. (C) BAT mRNA expression of genes encoding for browning markers of ctrl, 24 h fasted, and 4 h refed mice. (D) Ucp1 mRNA and (E) UCP1 protein expression (from WES digital western blotting) in BAT harvested after 1, 3, 6, 12, and 24 h of fasting with samples from circadian-matched ad libitum fed controls. GAPDH served as loading control. (F) Representative images (magnification, ×100) of BAT of ctrl and 24 h fasted mice stained with hematoxylin and eosin (E–H) and immunohistochemistry with anti-UCP1 antibody. (G) Cell size of was analyzed by using ImageJ and indicated as square pixels (px 2 ). (H) BAT weight in grams (g) of ctrl and 24 h fasted mice. (I,J) Respiratory exchange ratio (RER), (K,L) energy expenditure (EE), and (M,N) motility of ctrl and 24 h fasted mice as validated by indirect calorimetry in metabolic cages. Data are presented as mean values ± SEM. Significant differences in the mRNA expression of fed, fasted, and refed mice were analyzed by 2-way ANOVA and Tukey’s multiple comparisons test, for BAT weight, RER and energy expenditure unpaired t - test was performed. Differences not indicated with asterisks or indicated with ns are not statistically significant ( p > 0.05). *** p < 0.001, ** p < 0.01, and * p < 0.05.

Journal: Frontiers in Genetics

Article Title: p53 Regulates a miRNA-Fructose Transporter Axis in Brown Adipose Tissue Under Fasting

doi: 10.3389/fgene.2022.913030

Figure Lengend Snippet: Acute fasting-mediated alterations in BAT of mildly cold-stressed mice. (A) Number of up- and downregulated (1.5 x, FDR5) genes in BAT of 24 h fasted mice. (B) GSEA Hallmark analysis results with top five pathways with highest or lowest normalized enrichment scores (NES). Numbers of mapped genes are shown in the bars. (C) BAT mRNA expression of genes encoding for browning markers of ctrl, 24 h fasted, and 4 h refed mice. (D) Ucp1 mRNA and (E) UCP1 protein expression (from WES digital western blotting) in BAT harvested after 1, 3, 6, 12, and 24 h of fasting with samples from circadian-matched ad libitum fed controls. GAPDH served as loading control. (F) Representative images (magnification, ×100) of BAT of ctrl and 24 h fasted mice stained with hematoxylin and eosin (E–H) and immunohistochemistry with anti-UCP1 antibody. (G) Cell size of was analyzed by using ImageJ and indicated as square pixels (px 2 ). (H) BAT weight in grams (g) of ctrl and 24 h fasted mice. (I,J) Respiratory exchange ratio (RER), (K,L) energy expenditure (EE), and (M,N) motility of ctrl and 24 h fasted mice as validated by indirect calorimetry in metabolic cages. Data are presented as mean values ± SEM. Significant differences in the mRNA expression of fed, fasted, and refed mice were analyzed by 2-way ANOVA and Tukey’s multiple comparisons test, for BAT weight, RER and energy expenditure unpaired t - test was performed. Differences not indicated with asterisks or indicated with ns are not statistically significant ( p > 0.05). *** p < 0.001, ** p < 0.01, and * p < 0.05.

Article Snippet: Metabolic assessment of mice was performed using an indirect calorimetry system (TSE PhenoMaster, TSE Systems, Bad Homburg, Germany).

Techniques: Expressing, Western Blot, Control, Staining, Immunohistochemistry